Figure 6.
Summary of phosphorylation identifications. (A) Tetrameric structure (amino acids 43−372) of chicken Kir2.2 showing the transmembrane domain (TMD) and N- and C-terminal intracellular domains (PDB 3JYC).58 Phosphorylation sites were identified in blue (S313, Y326, and T347) and those previously reported but not identified (Y242 and S10) in green.47,70,71 S10, S13, S14, and S425 are not present in the truncated structures. The missing C-terminus is shown with a dashed line. (B) Intracellular domains (N-terminal amino acids 41−63 in orange and C-terminal amino acids 190−368 in gray) of mouse Kir2.1 (PDB 1U4F)59 in a similar orientation to (A) with only two of the four subunits shown for clarity. Missing N- and C-termini are shown by dashed lines with phosphorylation sites labeled. (C) Accessible surface area (ASA) for each residue calculated by ASAView72 for each structure. Sites listed are for Kir2.1 and are conserved between species except for Y326, which is a Phe in the Kir2.2 structure (see Figure S13). Distal N- and C-terminal residues are not in the structures, and so, the ASA is not available (n/a). Shaded areas are buried.