Figure 2.

Gating strategies. Firstly, we gated lymphocytes identified by CD45 from a leukocyte, then gated CD3⁺ T cells from a lymphocyte, and gated CD4⁺ and CD8⁺ T cells from CD3⁺ T cells. Secondly, we gated Trucont Beads. Thirdly, Tn (CD95^-CD45RO^-) and Tscm (CD95⁺CD45RO^-) were gated from the CD3⁺CD4⁺ CD45RA⁺CD62L⁺ T subpopulation. Tcm (CD95⁺CD45RO⁺) and Tem (CD95⁺CD45RO⁺) subpopulations were gated from CD3⁺CD4⁺CD45RA^-CD62L⁺ and CD3⁺CD4⁺CD45RA^-CD62L^- T subsets, respectively. Tte (CD95⁺CD45RO^-) subsets were gated from the CD3⁺CD4⁺CD45RA⁺CD62L^- T subpopulation. The gating logic for each subset of CD3⁺CD8⁺ T cell is the same as above. Tcm, central memory T cells, Tem, effector memory T cells, Tte, terminal effector T cells.