FIG. 4.

Expression of lecA::lux in P. aeruginosa. (A) P. aeruginosa lecA::lux was grown in LB medium in the absence of exogenously added AHL (○) or in the presence of 100 μM C4-HSL (■), C6-HSL (▴), or 3O-C12-HSL (⧫). RLU and OD₄₉₅ were determined as described in Materials and Methods. The results represent a single experiment, although the experiment was repeated three times with similar results. (B) Response of the P. aeruginosa lecA::lux fusion to C4-HSL, C6-HSL, and 3O-C12-HSL. Relative cumulative light units (RCU) were calculated as a percentage of the control (no exogenous AHL added) as described in Materials and Methods. For each AHL concentration, the experiment was repeated three times.