Fig. 5.

CCR2⁺ monocytes and monocyte-derived cells express immunoregulatory factors in the brain following T. spiralis infection. Single-cell RNA-seq was performed on sort-purified CD45^hi cells, from the brains of naive WT and infected (8 dpi) WT and DTR mice. (A) t-SNE dimension reduction illustrating distinct cell populations in the brain. Each group includes CD45^hi cells pooled from five mice. (B) The relative abundance of each cluster was determined in naive WT, infected WT mice, and infected DTR mice. (C and D) RNA from whole-brain tissue was extracted from WT, DTR, or STAT6KO mice on 8 dpi and evaluated via RT-qPCR. (E) Sort-purified microglia were pooled from naive and infected WT mice. Microglia from infected mice were incubated with rYM1 or rF10 and stimulated. TNF production was evaluated via ELISA. All panels are representative of at least three independent experiments each with at least three biological or technical replicates per group. Statistical analysis was performed using Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s., not significant. Error bars represent SD. Error bars represent ± SD. inf, T. spiralis-infected.