TABLE 3.
Representative amount of alkali required to alter a yeast suspension at an optical density at 600 nm of 0.4 from pH 6.5 to 10a
Designationb | Pertinent genotype | μmol of NaOH |
---|---|---|
a | BTN1+ HSP30+ BTN2+ | 1.7 |
b | btn1-Δ HSP30+ BTN2+ | 1.5 |
c | BTN1+ hsp30-Δ BTN2+ | 1.3 |
d | btn1-Δ hsp30-Δ BTN2+ | 1.2 |
e | BTN1+ HSP30+ btn2-Δ | 1.3 |
f | btn1-Δ HSP30+ btn2-Δ | 1.2 |
g | BTN1+ hsp30-Δ btn2-Δ | 1.3 |
h | btn1-Δ hsp30-Δ btn2-Δ | 1.1 |
Results reflect one of a series of five similar experiments, each of which resulted in a decreased buffering capacity for mutant strains compared to normal strains. The amount of HCl required to alter the pH of the same yeast strain suspension to from pH 6.5 to pH 3.0 was not significantly different.
See Table 1 for strain numbers.