Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Adenylation of LigTk with ATP or NAD+ as a cofactor. LigTk was incubated at 80°C for 2 h with [α-32P]ATP or [adenylate-32P]NAD+, both with 32P in the phosphate group of the AMP moieties. After incubation, the proteins were applied to SDS-PAGE gels and detected by autoradiography.
