Table 2.
Chemokine binding affinities (Kd, nM) of class A evasins (EVA-R, EVA-4, EVA-P, and EVA-1)
| Chemokines | Equilibrium dissociation constants Kd [nM] of WT evasins |
|||
|---|---|---|---|---|
| EVA-R | EVA-4 | EVA-P | EVA-1 | |
| CCL1 | - | 376 ± 63 | 15 ± 2 | - |
| CCL2 | - | 236 ± 132 | 4.1 ± 1.1 | - |
| CCL3 | 55.1 ± 2.5 | 0.2 ± 0.1 | 0.6 ± 0.1 | 0.21 ± 0.08 |
| CCL4 | 288 ± 141 | 158 ± 47 | 1.8 ± 0.1 | 60.7 ± 5.7 |
| CCL5 | - | 6.1 ± 0.1 | - | - |
| CCL7 | - | 0.8 ± 0.1 | 4.4 ± 0.3 | - |
| CCL8 | - | 0.4 ± 0.1 | 0.03 ± 0.01 | - |
| CCL11 | - | 20 ± 3 | 0.3 ± 0.1 | - |
| CCL13 | 84 ± 19 | 253 ± 17 | 0.5 ± 0.1 | - |
| CCL14 | 32.4 ± 4.9 | 1.1 ± 0.2 | 1.8 ± 0.6 | 92 ± 10 |
| CCL15 | 503 ± 166 | 240 ± 40 | 299 ± 244 | - |
| CCL16 | - | 4.1 ± 1.5 | 0.1 ± 0.1 | - |
| CCL17 | - | 283 ± 2 | 23 ± 15 | - |
| CCL18 | 651 ± 147 | 0.4 ± 0.1 | 0.6 ± 0.1 | 192 ± 27 |
| CCL22 | - | 8.5 ± 0.1 | 1.8 ± 0.1 | - |
| CCL23 | - | 7.4 ± 0.1 | - | - |
| CCL24 | - | 55 ± 2 | - | - |
The equilibrium dissociation constant (Kd) of four class A evasins for their cognate chemokines. A hyphen indicates nonmeasurable binding at 500 nM. The values are presented as mean ± SD obtained from three independent experiments (n = 3).