Table 3.
Thermal stability of chimeric evasins
| Evasins | Tm (°C) | |
|---|---|---|
| Parental evasin | EVA-R | 65.4 ± 0.4 |
| Chimeras containing the body of EVA-R | CHI-4R | 65.5 ± 0.1 |
| CHI-PR | 62.9 ± 0.4 | |
| CHI-1R | 64.1 ± 0.2 | |
| Parental evasin | EVA-4 | 79.9 ± 0.1 |
| Chimera containing the body of EVA-4 | CHI-R4 | 70.1 ± 0.2 |
| Parental evasin | EVA-P | 73.0 ± 0.1 |
| Chimeras containing the body of EVA-P | CHI-RP | 72.9 ± 0.1 |
| CHI-1P | 73.8 ± 0.1 | |
| Parental evasin | EVA-1 | 59.2 ± 0.1 |
| Chimeras containing the body of EVA-1 | CHI-R1 | 57.4 ± 0.6 |
| CHI-P1 | 58.6 ± 0.1 | |
Melting midpoint (Tm) of parental and chimeric evasins obtained from cooperative thermal unfolding curves. The values are presented as mean ± SD obtained from three independent experiments (n = 3).