FIGURE 3.

Species diversity of different brine samples revealed by Shannon index. The species diversity was shown using 16S rRNA-based high-throughput sequencing technology under culture-independent (A) and culture-dependent approaches (B). *p > 0.05, **p < 0.05. Archaea, archaeal 16S rRNA gene primers were used; Bacteria, bacterial 16S rRNA gene primers were used; AS-168 medium, colonies grown on the AS-168 medium were collected for the archaeal 16S rRNA-based high-throughput sequencing; NOM medium, colonies grown on the NOM medium were collected for the archaeal 16S rRNA-based high-throughput sequencing; C1-3, three samples collected in July 2021; C4-5, two samples collected in August 2018.