FIGURE 6.

Halophilic archaea and halotolerant bacteria isolated from brine samples. Cultivation of halophilic microbes from brine samples using the AS-168 agar plates (A). Cultures grown on the AS-168 and NOM agar plates were collected by washing with 20% (w/v) sterilized NaCl solution for archaeal 16S rRNA gene (V3 + V4) high-throughput sequencing (B). Microbial composition of randomly isolated strains identified by sequencing the almost complete 16S rRNA gene combined with sequence similarity search (C). C1-3, three brine samples including C1, C2, and C3 collected in July 2021; C4-5, two brine samples including C4 and C5 collected in August 2018.