Figure 5.

CDCA5 knockdown inhibits BC cellular stemness and growth in vivo. (A) mRNA and (B) protein levels of cancer stem cell markers in sh-CDCA5 and sh-NC BC cells were determined using reverse transcription-quantitative PCR and western blot analysis. (C) Representative images of mammospheres in sh-CDCA5 and sh-NC BC cells. The (D) number and (E) diameter of mammospheres from panel C was counted. CDCA5 knockdown inhibits BC tumor growth in vivo. (F) Volumes of MCF7 subcutaneous xenograft tumors were compared between the sh-CDCA5 and sh-NC groups (n=6). (G) Tumor weights were compared between the sh-CDCA5 and sh-NC groups (n=6). *P<0.05, **P<0.01 and ***P<0.001, vs. sh-NC. CDCA5, cell division cycle-associated 5; BC, breast cancer.