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. 2022 Sep 1;42(5):775–799. doi: 10.5851/kosfa.2022.e46

Table 3. Research on the development of cell culture media without using fetal bovine serum (FBS).

Species Cell type Media Additives for replace of FBS Results References
Bovine Chondrocytes Chemically defined medium (CDM) Transforming growth factor (TGF)-β3 The treatment group to which TGF-β3 was added without the addition of FBS showed excellent cell growth. Byers et al. (2008)
Dulbecco’s modified Eagle’s medium (DMEM) - The spontaneous calcium signaling in chondrocytes is affected by FBS exposure. Zhou et al. (2015)
High glucose DMEM - Cartilage tissue was formed through a three-dimensional culture system in a medium without serum and growth factors. Ahmed et al. (2014)
Corneal endothelium cells DMEM/F12 Platelet releasates Human platelet releasates used for ex vivo expansion of corneal endothelial cells. Chou et al. (2014)
Cumulus-oocyte complexes Modified synthetic oviduct fluid (mSOF) Insulin, transferrin, and selenium (ITS), bovine serum albumin (BSA) Investigation of the necessity and possible interactions of serum, cumulus cells, and oil during cell culture. Goovaerts et al. (2012)
Follicle McCoy’s 5a medium Insulin-like growth factor (IGF)-I (hrIGF-I) Investigation of culture and development of follicles through regulation of IGF-I bioavailability. Thomas et al. (2007)
Granulosa cells Modified alpha-minimum essential medium (MEM α) 0.1% polyvinyl alcohol Stimulation of progesterone production in granulosa cells through the addition of norepinephrine. Piccinato et al. (2012)
Keratinocytes (Comea) DMEM/F12 IGF-II and collagen expression in keratocytes. Kane et al. (2009)
Nucleus pulposus cells CDM TGF-β3 Comparison of cell development in serum-free/serum-supplemented media containing TGF-β3. Reza and Nicoll (2010)
Oocyte KSOM–PVA (potassium-supplemented simplex optimized medium-polyvinyl-alcohol) Glucose, fructose Effect of the addition of glucose and fructose to the supplemented serum-free medium on oocyte development. Bhuiyan et al. (2007)
McCoy’s 5a Activin, follicle-stimulating hormone Identification of ingredients that help the growth and activation of oocytes. McLaughlin and Telfer (2010)
Primary bovine satellite cells (BSCs) Beefy-9 - Confirmation of the effect of reducing the cost of cultured meat production by developing a serum-free medium. Stout et al. (2022)
Primary bovine myoblasts Seven commercial serum-free media LipoGroTM, XerumFreeTM Comparison of cell growth effects of 7 commercial serum-free media and 3 additives. Kolkmann et al. (2020)
Umbilical cord Wharton’s jelly cells Stemline® mesenchymal stem cell expansion medium - Comparison of cell growth and morphology in commercial serum-free medium. Cardoso et al. (2012)
Whole embryo HEPES buffered TCM 199 medium Growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF), recombinant albumin (RA), hyaluronan (HA) Development of fetal calf serum or BSA free medium using various growth factors and cytokines or other molecules with surfactant and embryotrophic properties. Moreno et al. (2015)
Two-step defined culture system (C1/C2 medium) - Development of a defined culture medium that supported improved in vitro bovine embryo development and calving rate after embryo transfer. Lim et al. (2007)
- Evaluation of the developmental competence of bovine embryos in the serum-free medium at early or later embryonic stages. Park et al. (2010)
mSOF CDM, BSA Comparison of blastocyst development in embryos cultured in serum-supplemented and serum-free media. Jang et al. (2011)
Dulbecco’s phosphate buffered saline-sericin (cryopreservation) Silk protein sericin Effect of silk protein sericin in serum-free freezing of bovine embryos. Isobe et al. (2013)
TCM-199 medium Albumin, ITS Evaluation of the development of bovine embryos and blastocyst quality in serum-free conditions. Wydooghe et al. (2014)
Porcine Bone marrow-derived mesenchymal stromal cells Ultra-CULTURE Epithelial growth factor (EGF) Confirmation of substitutability of the serum-free medium through the addition of growth factors. Wang et al. (2013)
Chondrocytes Ham F12 Autologous serum, fibroblast growth factor—basic (FGF-basic), IGF-1 Indentification of serum replacement effect of autologous serum and growth factors in serum-free medium. Kamil et al. (2007)
Hepatocytes DMEM/199 medium Dimethyl sulfoxide (DMSO), dexamethasone Verification of cell monolayer formation and maintenance in serum-free medium condition. Caperna et al. (2011)
Williams E medium - Comparison of phenotype-induced changes in cells cultured in serum-free/serum-supplemented media. Rasmussen (2017)
Oocyte Modified porcine oocyte medium (mPOM) - Defined system for in vitro production of porcine embryos. Yoshioka et al. (2008)
In vitro production studies of pig embryos in a defined serum-free medium. Akaki et al. (2009)
- In vitro-derived blastocyst quality improvement study in a defined serum-free medium. Koike et al. (2010)
- Study of interaction between oocytes during in vitro maturation in a defined serum-free medium. Appeltant et al. (2015)
Modified medium 199 - Developmental capacity of selected oocytes to mature in a defined serum-free medium. Ishizaki et al. (2009)
North Carolina State University-23 (NCSU-23) Modified NCSU-23 medium - Effect of amino acid addition on maturation, fertilization, and preimplantation development of oocytes. Hong and Lee (2007)
Whole embryo Porcine zygote medium (PZM)-5 Defined system for in vitro production of porcine embryos. Yoshioka et al. (2008)
PZM-4 Subsequent embryo development in a defined serum-free medium using selected oocytes. Ishizaki et al. (2009)
PZM-3/4/5 Effects of the CDM on the early development of porcine embryos. Cao et al. (2012)
Glucose, glycine The effects of glucose and/or glycine on the in vitro development of porcine blastocysts. Mito et al. (2012)
Porcine blastocyst medium, PZM-5 Birth of piglets from in vitro-produced porcine blastocysts vitrified and warmed in a chemically defined medium. Mito et al. (2015)
TCM-199–HEPES (TCM) Polyvinyl alcohol Vitrification and warming of in vivo-derived porcine embryos in a chemically defined medium. Sanchez-Osorio et al. (2010)
Chicken PBS-12SF (original CHCC-OU2 line) OptiPROTM SFM - Evaluation of immortalized chick embryo cell lines for vaccine production in serum-free conditions. Coussens et al. (2011)
Duck AGE1.CR, AGE1. CR.pIX SFM-G, HyQ®SFM4MegaVirTM - Evaluation of the growth of avian designer cell lines in serum-free media for use in vaccine manufacturing. Lohr et al. (2009)
Sheep Oocyte TCM 199 Sericin, polyvinyl alcohol Confirmation of FBS replacement effect of sericin. Hajarian et al. (2017)
HEPES buffered TCM 199 Growth factor, hormones Comparison of oocyte development in serum-free medium supplemented with growth factors and hormones. Arunakumari et al. (2010)
Goat Whole embryo mSOF, DI / II Bovine serum albumin, polyvinyl alcohol Identification of optimal medium conditions and cryotolerance levels for culturing goat embryos. Choi et al. (2016)