Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 6;48(4):181. doi: 10.3892/or.2022.8396

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 5. — Knockdown of ClC-3 expression in A549 cells prevents PTX resistance induced by upregulation of SOX2 expression. (A) (Left) Protein expression levels of MDR1, ABCC2, ABCC10 and tubulin in A549 cells transfected with ClC-3 plasmid or siSOX2 and its control vector or siNC. (Right) Protein expression was semi-quantified using ImageJ. (B) Viability was examined using a CCK-8 assay in A549 cells transfected with ClC-3 plasmid or siSOX2 and its control vector or siNC. Cells were treated with PTX for 48 h. (C) (Left) Protein expression levels of MDR1, ABCC2, ABCC10 and tubulin in A549 cells transfected with SOX2 plasmid or siClC-3 and its control vector or siNC. (Right) Protein expression was semi-quantified using ImageJ. (D) Viability was examined using a CCK-8 assay in A549 cells transfected with SOX2 plasmid or siClC-3 and its control vector or siNC. Cells were treated with PTX for 48 h. Tubulin were used as a loading control in western blotting. All data are presented as the mean ± standard deviation. ^**P<0.005, ^***P<0.001. ns, not significant. Relative, vs. respective control. ClC-3, chloride voltage-gated channel 3; A549-PTX cells, paclitaxel-resistant A549 non-small cell lung cancer cells; PTX, paclitaxel; MDR1, multidrug resistance mutation 1; ABCC2, ATP binding cassette subfamily C member 2; ABCC10, ATP binding cassette subfamily C member 10; siRNA/si, small interfering RNA; CCK-8, Cell Counting Kit-8.