FIGURE 1.

Impact of resveratrol or/and ASO against β1-integrin on TME-mediated cell morphology and expression of E-cadherin and paxillin in CRC cells. HCT116 (A) and RKO (B) cells were grown on coverslips in TME, left untreated or treated with increasing doses of resveratrol (1, 2, 5 µM) or were transfected with β1-SO or β1-ASO as outlined in Material and Methods. After rinsing with phosphate-buffered water, the cell morphology of CRC cells was examined by phase contrast (A,B), (top row each). Immunolabeling (red) with anti-E-cadherin (A,B), (middle row each) or paxillin (A,B), (bottom row each) was observed by immunofluorescence microscopy. Special attention is paid to the discovered resveratrol-induced plaque-like deposits, highlighted with black (A,B), (top row each) and white (A,B), (middle row each) arrows. Statistical analysis: Plaque-like deposits were quantified by couting in 20 different microscopiy fields. Related to TME control, *p < 0.05 and **p < 0.01. Microscope: Leica DM 2000. Magnification ×600; scale bar = 30 µm. Inset magnification: ×1200; scale bar = 15 µm.