FIGURE 2.

Impact of resveratrol or/and ASO against β1-integrin on TME-mediated activation and nuclear translocation of p65-NF-κB in CRC cells. HCT116 (A,B) and RKO (B) cells in TME on cover glasses without treatment or only treated with resveratrol (5 µM) or resveratrol-treated (5 µM) with addition of β1-SO (0.5 µM) or knocked down with β1-ASO (0.1, 0.2, 0.5 µM). Anti-phosho-NF-κB immunolabeled (red) and DAPI-stained (blue). White arrows = p65-NF-κB positive CRC cells. Microscope: Leica DM 2000. Magnification ×600; scale bar = 30 µm. Statistical evaluation: *p < 0.05 and **p < 0.01, compared to TME control.