Figure 5.

The gene-cleaving effect of CRISPR/Cas9_-3NLS/sgHMGA2@PDA in vitro (A) Alignment of five representative colony PCR sequences derived from the cells co-incubated with CRISPR/Cas9_-3NLS@PDA for 24 h to the supposed sequence. (B) Alignment of five representative colony PCR sequences derived from the cells co-incubated with CRISPR/Cas9_-3NLS/sgHMGA2@PDA for 24 h to the supposed sequence; the region between the purple boxes represents the sequence of the plasmid backbone; the region between the red boxes represents the sequences of the inserted PCR fragment; green boxes: cloning site; the blue and yellow regions represent the sequence encoding sg-HMGA2-1, sg-HMGA2-2, and sg-HMGA2-3 *: the range between two asterisk represents 10bp.