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. 2022 Sep 2;12:978533. doi: 10.3389/fonc.2022.978533

Table 2.

The cleavage efficiency calculation of CRISPR/Cas9-3NLS/sgHMGA2@PDA.

Time after co-incubation Group No. of total clones No. of cleavage clones No. of no cleavage clones Cleavage rate (%)
24 h CRISPR/Cas9-3NLS@PDA 20 0 20 0
CRISPR/Cas9-3NLS/sgHMGA2@PDA 74 51 ≦200 bp: 6 23 68.9
200-300 bp: 23
≧300 bp: 22
48 h CRISPR/Cas9-3NLS@PDA 20 0 20 0
CRISPR/Cas9-3NLS/sgHMGA2@PDA 36 29 ≦200 bp: 4 7 80.55
200-300 bp: 18
≧300 bp: 7
72 h CRISPR/Cas9-3NLS@PDA 20 0 20 0
CRISPR/Cas9-3NLS/sgHMGA2@PDA 50 41 ≦200 bp: 14 9 82
200-300 bp: 16
≧300 bp: 11