FIG. 2.

Toluene molecules adhering to IH-2000 (●) or strain 32 (▴) cells or MVs (■) and the amount of fatty acids in MVs (□) in the culture. Toluene was added to a culture of strain IH-2000 or 32 (time = 0). Then the amount of toluene molecules adhering to the cells was measured, and the amount per fatty acid chain was calculated because the MVs were found to be composed of phospholipids and LPS. Error bars show means ± SDs (n = 3). The MV fractions were periodically prepared as described in Materials and Methods from the culture of IH-2000.