TABLE 1.
Fatty acid compositiona of MVs
| Fatty acidb | Originc | Amt of lipids
|
|
|---|---|---|---|
| nmol/ml of culture | Ratio (%) | ||
| C10:0-3OH | LPS | 0.100 | 6.1 |
| C12:0 | LPS | 0.161 | 3.8 |
| C12:0-2OH | LPS | 0.437 | 16.6 |
| C12:0-3OH | LPS | 0.585 | 6.2 |
| C16:0 | PL | 0.468 | 22.2 |
| C16:1t | PL | 0.365 | 17.8 |
| C16:1 | PL | 0.163 | 13.8 |
| C18:0 | PL | 0.033 | 1.2 |
| C18:1t | PL | 0.116 | 4.4 |
| C18:1 | PL | 0.210 | 7.9 |
| Total | 2.64 | 100 | |
a
The samples of freeze-dried MVs or bacterial cells were heated in 5% (wt/vol) methanolic HCl at 100°C for 3 h. The resulting FAMEs were extracted twice with n-hexane and concentrated under a stream of nitrogen gas. The FAMEs were analyzed using a gas-liquid chromatograph (model GC-380; GL-Science).
b
C10:0-3OH, 3-hydroxyldecanoic acid; C12:0, dodecanoic acid; C12:0-2OH, 2-hydroxyldodecanoic acid; C12:0-3OH, 3-hydroxyldodecanoic acid; C16:0, hexadecanoic acid; C16:1t, trans-hexadecanoic acid; C16:1, cis-hexadecanoic acid; C18:0, octadecanoic acid; C18:1t, trans-octadecanoic acid; C18:1, cis-octadecanoic acid.
c
These fatty acid molecules were found in the membrane of IH-2000 (17). PL, phospholipid.