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. 2022 Sep 16;41:275. doi: 10.1186/s13046-022-02475-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — ETV1 transcriptionally upregulates PTK2 and c-MET expression. (A) Human Liver Cancer PCR Array was employed for detecting the upregulated genes in PLC/PRF/5-ETV1 and down-regulated genes in MHCC97H-shETV1 cells compared with controls (fold change ≥ 2.0). Overlapping genes were shown in the Venn diagram. (B-C) Compared with the controls, PTK2 and c-MET were upregulated when ETV1 was overexpressed in PLC/PRF/5 cells, and they were down-regulated when ETV1 was knocked down in MHCC97H cells, detected by qRT-PCR and western blotting. (D) Relative luciferase activity of PTK2 and MET promoter reporter vectors after cotransfection of pCMV-ETV1 and PTK2 or MET plasmid constructs in PLC/PRF/5 cells. (E-F) Relative luciferase activity of indicated PLC/PFR/5 cells cotransfected with pCMV-ETV1 and truncated or mutated luciferase constructs. (G-H) ChIP assay of ETV1 binding to the PTK2 or MET promoter in PLC/PRF/5 cells, as well as HCC specimens. * Represented p < 0.05. All data were displayed as Mean ± SD