Fig. 2.

Disruption of myeloid-specific Notch1 exacerbates IR-triggered hepatocyte death. The Notch1^FL/FL and Notch1^M−KO mice were subjected to 90 min of partial liver warm ischemia, followed by 6 h of reperfusion. A TUNEL staining in ischemic livers (n = 4–6 mice/group). Scale bars, 100 μm. B Western blot analysis of Bax, Bcl-2, and cleaved caspase-3. Representative of three experiments. C Immunohistochemistry staining of cleaved caspase-3 in ischemic livers (n = 4–6 mice/group). Quantification of c-caspase3 positive cells, Scale bars, 40 μm. D Western blot analysis of TRAF6, p-TAK1, p-P65, RIPK3 and p-MLKL in Notch1^FL/FL and Notch1^M−KO mice. Representative of three experiments. E Immunohistochemistry staining of RIPK3 in ischemic livers (n = 4–6 mice/group). Scale bars, 40 μm. All data represent the mean ± SD. **p < 0.01