Fig. 3.

β-catenin is essential for the macrophage Notch1-mediated inflammatory response in liver IRI. The Notch1^M−KO mice were injected via tail vein with bone marrow-derived macrophages (BMMs, 5 × 10⁶ cells/mouse) transfected with lentivirus expressing β-catenin (Lv-β-catenin) or GFP control (Lv-GFP) 24 h prior to ischemia. A Representative histological staining (H&E) of ischemic liver tissue (n = 4–6 mice/group) and Suzuki’s histological score. Scale bars, 200 μm. B Liver function in serum samples was evaluated by serum ALT and AST levels (IU/L) (n = 4–6 samples/group). C Immunofluorescence staining of CD11b⁺ macrophages in ischemic livers (n = 4–6 mice/group). Quantification of CD11b⁺ macrophages, Scale bars, 40 μm. D Immunohistochemistry staining of Ly6G⁺ neutrophils in ischemic livers (n = 4–6 mice/group). Quantification of Ly6G⁺ neutrophils, Scale bars, 40 μm. E Quantitative RT-PCR analysis of TNF-α, IL-1β, CCL-2 and CXCL-10 mRNA levels in ischemic livers (n = 3–4 samples/group). F Western blot analysis and relative density ratio of TRAF6, p-TAK1, and p-P65. Representative of three experiments. All data represent the mean ± SD. *p < 0.05. **p < 0.01