Fig. 4.

Myeloid Notch1 signaling controls immune regulation and hepatocyte necroptosis in a RIPK3-dependent manner in IR-stressed liver. The Notch1^M−KO mice were injected via tail vein with RIPK3 siRNA (2 mg/kg) or non-specific (NS) control siRNA mixed with mannose-conjugated polymers at 4 h prior to ischemia. A Representative histological staining (H&E) of ischemic liver tissue (n = 4–6 mice/group) and Suzuki’s histological score. Scale bars, 200 μm. B Liver function in serum samples was evaluated by serum ALT and AST levels (IU/L) (n = 4–6 samples/group). C Immunofluorescence staining of CD11b⁺ macrophages in ischemic livers (n = 4–6 mice/group). Quantification of CD11b⁺ macrophages, Scale bars, 40 μm. D Immunohistochemistry staining of Ly6G⁺ neutrophils in ischemic livers (n = 4–6 mice/group). Quantification of Ly6G⁺ neutrophils, Scale bars, 100 μm. E Quantitative RT-PCR analysis of TNF-α, IL-1β, CCL-2 and CXCL-10 mRNA levels in ischemic livers (n = 3–4 samples/group). F TUNEL staining in ischemic livers (n = 4–6 mice/group). Results were scored semi-quantitatively by averaging the number of necroptotic cells. All data represent the mean ± SD. *p < 0.05. **p < 0.01