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. 2022 Sep 15;4:53. doi: 10.1186/s42523-022-00203-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Differential abundance analysis between starved and fed fish in PC samples (a) and MG samples (b). Important genera were determined with random forest classification. Mean decrease gini served as indicator value. c Shows the source tracking analysis determined by the FEAST framework. Bars show the average contribution of each source (either feed, water or unknown) to the overall microbial community for different gut compartments, feeding status and timepoints. d Illustrates a principal coordinates analysis (PCoA) derived from weighted UniFrac distances between environmental samples (MW = Marine water and feed), starved fish ((48 h feed withdrawal) and recently fed fish at two different points in time (T0 and T1). T1 was sampled 14 days after T0. Lines mark the position of the centroids of each group. For illustration purposes no differentiation between PC and MG samples was made. However, differentiation was included in Permanova calculations (Additional file 1: Tables S7, S8)