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. 2022 Sep 2;15:976349. doi: 10.3389/fnmol.2022.976349

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Copyright © 2022 Tsuneoka, Atsumi, Makanae, Yashiro and Funato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rapid quenching of autofluorescence in the mouse cingulate cortex by photobleaching using TiYO^TM. (A) Change in autofluorescence according to the duration of photobleaching. Each row shows photos from different specimens. Scale bar: 50 μm. (B) The density of lipofuscin-like granules. (C) Maximum fluorescence intensity. (D) Total fluorescence intensity. (B–D) Dots and lines represent the measured values and their means, respectively. Fluorescence intensities are shown as arbitrary units (A.U.). (E,F) Orthogonal views of the same 40 μm-thick section before (E) and after photobleaching (F).