Skip to main content
. 2022 Sep 2;15:976349. doi: 10.3389/fnmol.2022.976349

Figure 5.

Figure 5

Schematic illustration describing two rounds of ISH combined with IHC. First, all probes of mRNAs of interest were hybridized simultaneously. The tissue was subjected to two rounds of staining by HCR for three mRNAs, confocal imaging, and photobleaching. Then, the tissue was subjected to IHC and confocal imaging.