Table 1.
Sequences of primers applied for quantitative real-time PCR analysis in gibel carp.
| Gene name | prime sequence | Product size (bp) | Gene bankaccession No. |
|---|---|---|---|
| Claudin 4 | F: GCCGGTGTGATTTTCATCGTT | 108 | XM_026234420.1 |
| R: CTGTGCTTGGTTCAACAAGGG | |||
| Interleukin-10 (il-10) | F: AGCCATGGGAGAGCTTGATA | 227 | XM_026275831.1 |
| R: ATGATGACGTGCAAGCGTT | |||
| Apoptosis regulator Bcl-2 (bcl2) | F: AAAGGATGTACCAGCGCGAA | 83 | XM_026237836.1 |
| R: GGCTAAGAATCTGCGTTGCG | |||
| Caspase 3 (casp3) | F: ATCATGACCAGGGTCAACCA | 119 | XM_026266756.1 |
| R: TACATCTCTTTGGTGAGCAT | |||
| Endoplasmic reticulum oxidoreductase 1 alpha (ero1α) | F: ATGCCCAACACAAGCAACAC | 129 | XM_026242578.1 |
| R: TGACAACAGCGACCGAAAGT | |||
| Peroxiredoxin (prx) | F: AGGTCATCGCTGCTTCCACCG | 90 | XM_026211451 |
| R: TGTTCATGGAGCCCAGGCCAC | |||
| CNC homolog 1 (bach1) | F: TGGAGCGCAGGAGCTTTCGAG | 98 | XM_026282740 |
| R: AGTGGGGTTTGGTCGGCTGTG | |||
| Superoxude dismutase (sod) | F: GTCCGCACTACAACCCTCAT | 134 | JQ776518.1 |
| R: GGTCACCATTTTATCCACAA | |||
| Catalase (cat) | F: CTCCAACGGCAACTTCCCAT | 102 | JX477239.1 |
| R: CACACCTTAGTCAAATCAAA | |||
| Heat shock protein 70 (hsp70) | F: CTCAACAAGAGCATCAACCCAG | 155 | JN006055.1 |
| R: ATGACTCCACCAGCCGTTTC |