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. 2022 Sep 2;13:937741. doi: 10.3389/fphar.2022.937741

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Copyright © 2022 Johnson, Doucette, Edwards, Verdi, McFarland, Hulke, Fowler, Watts and Klein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intrathecal injection of small interfering RNA sequences targeting AC1 reduces Adcy1 mRNA in spinal cord and DRG of mice. (A) Significant reduction of Adcy1 mRNA copies present in mice injected with AAV9-ADCY1-shRNA-GFP (Adcy1) vs. AAV9-Scrambl-shRNA-GFP (Scramble; unpaired t-tests, *p < 0.05). No significant changes are seen in Adcy5 (B), Adcy8 (C), or Oprm1 (D) mRNA expression. n = 8–10/group. (E) Protein levels of AC1 collected from spinal cord are lower in AAV9-ADCY1-shRNA-GFP (Adcy1) vs. AAV9-Scrambl-shRNA-GFP injected mice (unpaired t-test, p < 0.0001), n = 4–5/group. Injections were verified by Immunofluorescence analysis in the spinal cord (F), DRG (G), and sciatic nerves (H). Scale bar = 200uM. Data presented as mean ± SEM.