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. 2022 Sep 2;13:937741. doi: 10.3389/fphar.2022.937741

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Copyright © 2022 Johnson, Doucette, Edwards, Verdi, McFarland, Hulke, Fowler, Watts and Klein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intrathecal delivery of AAV9-Adcy1-shRNA reduces mechanical hypersensitivity following intraplantar CFA. Mechanical paw withdrawal thresholds were measured before and after a unilateral hind paw injection of 20 µl CFA. (A) AAV9-Adcy1 (●) mice had significantly higher MPW thresholds than AAV9-scramble mice (□) on the injected paw (repeated measures ANOVA with Bonferroni’s post hoc test, F (1, 18) = 6.157, p = 0.0232), (B) and on the CFA uninjected paw (repeated measures ANOVA with Bonferroni’s post hoc test, F (1, 18) = 9.148, p = 0.0073). (C) AAV9-Adcy1 mice have increased burrowing (unpaired t-test, p = 0.0188) (D) and higher nest building scores compared to control mice (Mann Whitney test, p < 0.0001). Asterisk indicates statistical significance at each individual time point (p < 0.05), n = 10/group. Data in A-C presented as mean ± SEM, data in D presented as median ± 95% confidence interval.