TABLE 2.

Gene specific primers used for qRT-PCR. The NCBI gene accession number, resulting base pair length, and both the forward and reverse primers for each gene for qRT-PCR analysis.

Gene	NCBI number	Length	Forward	Reverse
18s	NR_003278.3	149bp	5′-CGC​CGC​TAG​AAG​TGA​AAT​TCT​T-3′	5′-CAG​TCG​GCA​TCG​TTT​ATG​GTC-3′
Adcy1	NM_009622.1	115bp	5′-TGC​AGA​CAT​CGT​GGG​TTT​CA-3′	5′-ACA​GTG​GTT​TTC​GGC​TA-3′
Adcy2	NM_153534.2	140bp	5′-CTA​AAC​CGA​GTG​CTG​CTG​GA-3′	5′-TTG​AAG​TCC​GGA​ATG​GAG​GC-3′
Adcy3	NM_138305.3	199bp	5′-TCT​GGG​GTC​CAA​GAA​GAG​AGA-3′	5′-GAC​CCG​GAA​TTT​GGG​ATT​GTC-3′
Adcy5	NM_001012765.4	151bp	5′-TGA​TCG​AGG​CCA​TCT​CGT​TG-3′	5′-TGG​TTG​GCC​AGA​GTG​ACA​TC-3′
Adcy6	NM_007405.2	162bp	5′-TGC​GGT​GAG​GGA​GAA​TCA​CT-3′	5′-ACA​CCT​GTT​ACC​TCA​CGC​AC-3′
Adcy8	NM_001291903.1	191bp	5′-CCG​CAT​CTA​CAT​CCA​TCG​CT-3′	5′-AGT​AGT​AGC​AGT​CCC​CCA​GG-3′
Prkaca	NM_008854.5	96bp	5′-TTT​GCC​AAG​CGT​GTG​AAA​GG-3′	5′-AGC​CTT​GTT​GTA​GCC​TTT​GCT-3′
Prkacb	NM_011100.4	122bp	5′-TGC​AGC​CCA​GAT​TGT​GCT​AA-3′	5′-ACC​CGA​AAT​CTG​TGA​CCT​GG-3′
Rapgef3	NM_001177810.1	145bp	5′-GGA​AGT​GCA​TGA​GCT​GAC​CC-3′	5′-CAC​CTG​GTG​GAT​CCT​GTT​GAA​G-3′
Rapgef4	NM_001204165.1	96bp	5′-TCC​AAG​AGC​TGC​CTC​CAT​TG-3′	5′-GAA​TCA​ACG​TCC​CTC​AGA​AT-3′
Gapdh	NM_001289726.1	85bp	5′-TGA​CCT​CAA​CTA​CAT​GGT​CTA​CA-3′	5′-CTT​CCC​ATT​CTC​GGC​CTT​G-3′