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. 2022 Aug 11;35(4):ivac210. doi: 10.1093/icvts/ivac210

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© The Author(s) 2022. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3: — Circ_0002194 could bind to microRNA-637 (miR-637). (A) The miR-637 was quantified using reverse transcription-quantitative polymerase chain reaction in 0 μg/ml, 25 μg/ml, 50 μg/ml or 100 μg/ml oxidized low-density lipoprotein treatment groups. (B) The binding sites between circ_0002194 and miR-637 were predicted by CircInteractome. (C-D) The interaction between circ_0002194 and miR-637 was determined by the Imprint RNA Immunoprecipitation assay in human umbilical vein endothelial cells (C) and the dual-luciferase reporter assay in 293 T cells (D). *P < 0.05. Ago 2: Argonaute-2; circ: circular; IgG: immunoglobin G; miR-637: microRNA-637; mut: mutant-type; NC: negative control; wt: wild-type.