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. 2022 Aug 11;35(4):ivac210. doi: 10.1093/icvts/ivac210

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© The Author(s) 2022. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5: — The expression of phosphofurin acidic cluster sorting protein 2 (PACS2) was mediated by the circ_0002194/microRNA-637 (miR-637) axis. (A) The PACS2 protein level was measured by the Western blot after treatment with 0 μg/ml, 25 μg/ml, 50 μg/ml and 100 μg/ml oxidized low-density lipoprotein. (B-C) The binding between PACS2 and miR-637 was predicted by Targetscan (B) and affirmed by the dual-luciferase reporter assay in 293 T cells (C). (D) The Western blot was applied to examine the protein expression of PACS2 in the small-interfering (si)-negative control, si-circ_0002194#1, si-circ_0002194#1+inhibitor negative control or the si-circ_0002194#1+miR-637 inhibitor group. *P < 0.05. GAPDH: oxidized low-density lipoprotein; miR-637: microRNA-637; mut: mutant-type; NC: negative control; OX-LDL: oxidized low-density lipoprotein; PACS2: phosphofurin acidic cluster sorting protein 2; si: small interfering; 3'UTR: three prime untranslated region; wt: wild-type.