FIG. 2.

Accumulation of CA by JCM 1044 at different external pH values. The experiments were carried out at different pH values: 7.6 (◊), 7.3 (▵), 7.0 (●), 6.6 (▴), and 6.2 (⧫). For energization of the cells, glucose was added at t = 15 min. CA amounts in the nonenergized cells are demonstrated by only one line (■), since these were very similar at all pH values tested. CA* denotes both cholic acid and cholate anion.