KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-ABCB5 (clone 3C2-1D12) | Frank lab | PMID: 25030174 |
| Mouse monoclonal anti-CD45-PE | BioLegend | 368510 Cat# 368510; RRID:AB_2566370 |
| Mouse monoclonal anti-CD45- Brilliant Violet 421 | BioLegend | 368522 Cat# 368522; RRID:AB_2687375 |
| Human monoclonal anti-BCAM-VioBright FITC | Miltenyi Biotec | 130-104-839 Cat# 130-104-839; RRID:AB_2656519 |
| Rabbit polyclonal anti-BCAM | NOVUS Biologicals | NBP2-31994 Cat# NBP2-31994: RRID:AB_2922815 |
| Mouse monoclonal anti- Integrin β4 | NOVUS Biologicals | NB100-65599 Cat# NB100-65599; RRID:AB_959522 |
| Mouse monoclonal anti-KRT12 | Santa Cruz Biotechnology | sc-515882 Cat# sc-515882; RRID:AB_2922816 |
| Rabbit monoclonal anti-KRT12 | Abcam | ab185627 Cat# ab185627; RRID:AB_2889825 |
| Mouse monoclonal anti-NUMB | Santa Cruz Biotechnology | sc-136554 Cat# sc-136554; RRID:AB_10611794 |
| Mouse monoclonal anti-MUC16 | Abcam | ab1107 Cat# ab1107; RRID:AB_297721 |
| Mouse monoclonal anti-laminin α5 | Atlas Antibodies | AMAb91124 Car# AMAb91124; RRID:AB_2665809 |
| Rabbit polyclonal anti-laminin α5 | GeneTex | GTX55867 Cat# GTX55867; RRID:AB_2887940 |
| Mouse monoclonal anti-p63 | Abcam | ab735 Cat# ab735; RRID:AB_305870 |
| Rabbit monoclonal anti-p63 | Abcam | ab124762 Cat# ab124762; RRID:AB_10971840 |
| Rabbit polyclonal anti-β-actin | Cell Signaling Technology | 8457L Cat# 8457; RRID:AB_10950489 |
| Donkey anti-Mouse IgG- Alexa Fluor 488 | Thermo Fisher Scientific | A21202 Cat# A21202; RRID:AB_141607 |
| Donkey anti-Rabbit IgG- Alexa Fluor 568 | Thermo Fisher Scientific | A10042 Cat# A10042; RRID:AB_2534017 |
| Anti-mouse IgG, HRP-linked Antibody | Cell Signaling Technology | 7076S Cat# 7076; RRID:AB_330924 |
| Anti-rabbit IgG, HRP-linked Antibody | Cell Signaling Technology | 7074S Cat# 7074; RRID:AB_2099233 |
| Bacterial and virus strains | ||
| deltaNp63alpha-FLAG | Addgene | 26979 |
| BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase | This manuscript | 188391 |
| Biological samples | ||
| Human whole globes and corneas | Saving Sight (Kansas City, MO) andN/A CorneaGen (Seattle, WA) eye banks | |
| Chemicals, peptides, and recombinant proteins | ||
| PluriSTEM Dispase II Solution | MilliporeSigma | SCM133 |
| TrypLE™ Express Enzyme | Thermo Fisher Scientific | 12605036 |
| DMEM/F-12 | Thermo Fisher Scientific | 11320033 |
| Recombinant Human KGF (FGF-7) | PeproTech | 100-19 |
| Y-27632 dihydrochloride | TOCRIS | 1254 |
| B-27 Supplement (50X), serum free | Thermo Fisher Scientific | 17504001 |
| DMEM, high glucose, pyruvate | Thermo Fisher Scientific | 11995-065 |
| Fetal Bovine Serum (FBS) | Thermo Fisher Scientific | 10438026 |
| HyClone Phosphate Buffered Saline solution | GE Healthcare | SH30256.01 |
| SYTOX Green Dead Cell Stain | Thermo Fisher Scientific | S34860 |
| Propidium Iodide Staining Solution | BD Biosciences | 556463 |
| GloCell™ Fixable Viability Dye Violet 450 | STEMCELL Technologies | 75009 |
| Bovine serum albumin (BSA) | MilliporeSigma | A7030 |
| 10% neutral buffered formalin | Fisher Scientific | SF100-4 |
| TissueTek® O.C.T Compound | Sakura | 4583 |
| 16% paraformaldehyde | Electron Microscopy Sciences | 15710 |
| Normal donkey serum | Jackson Immuno Research Laboratories | 017-000-121 |
| Triton™ X-100 | MilliporeSigma | X-100 |
| Tris-Buffered Saline (TBS) | Boston BioProducts | BM301 |
| Hoechst 33342 | Thermo Fisher Scientific | H3570 |
| ProLong Gold Antifade Mountant | Thermo Fisger Scientific | P36934 |
| TaqMan™ Fast Universal PCR Master Mix | Thermo Fisher Scientific | 4366072 |
| Mitomycin C from Streptomyces caespitosus | MilliporeSigma | M4287 |
| DMEM, high glucose, pyruvate, no glutamine | Thermo Fisher Scientific | 10313021 |
| Ham’s F-12 Nutrient Mix | Thermo Fisher Scientific | 11765054 |
| Hydrocortisone hydrogen succinate | MilliporeSigma | BP187 |
| 3,3,5-Triiodo-L-thyronine sodium salt | MilliporeSigma | T2752 |
| Transferrin, Bovine (Holo form), lyophilized | Thermo Fisher Scientific | 11107047 |
| L-glutamine | Thermo Fisher Scientific | 25030081 |
| Insulin-Transferrin-Selenium (ITS-G) (100X) | Thermo Fisher Scientific | 41400045 |
| Cholera Toxin (AZIDE-FREE) from Vibrio cholerae | List Biological Laboratories | 100B |
| HyClone Penicillin-Streptomycin 100X solution | GE Healthcare | SV30010 |
| Rhodamine B | MilliporeSigma | R6626 |
| iMatrix-511 | Nacalai Tesque | 892012 |
| RIPA buffer | Cell Signaling Technology | 9806S |
| cOmplete™ Protease Inhibitor Cocktail | MilliporeSigma | 11873580001 |
| SDS-sample buffer | Boston BioProducts | BP111NR |
| 2-mercaptoethanol | MilliporeSigma | M3148 |
| PVDF Blotting Membrane | GE healthcare | 10600023 |
| Blotting-Grade Blocker | Bio-Rad | 1706404 |
| Tween 20 | MilliporeSigma | P1379 |
| Western Lightning Plus-ECL | PerkinElmer | NEL104001EA |
| Lipofectamine™ RNAiMAX Transfection Reagent | Thermo Fisher Scientific | 13778075 |
| Lipofectamine™ 3000 Transfection Reagent | Thermo Fisher Scientific | L3000008 |
| PowerSYBR Green PCR Master Mix | Thermo Fisher Scientific | 4367659 |
| Critical commercial assays | ||
| Alexa Fluor™ 647 Antibody Labeling Kit | Thermo Fisher Scientific | A20186 |
| Chromium Single Cell B Chip Kit | 10X Genomics | PN-1000073 |
| Chromium Single Cell 3’ GEM, Library & Gel Bead Kit v310X Genomics | PN-1000075 | |
| RNeasy plus Mini Kit | QIAGEN | 74136 |
| DNA-free™ DNA Removal Kit | Thermo Fisher Scientific | AM1906 |
| High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | 4368814 |
| Hematoxylin and Eosin Stain Kit | Vector Laboratories | H-3502 |
| OrisTM Cell Migration Assay Kit | Platypus Technologies | CMA1.101 |
| EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit | MilliporeSigma | 17-10086 |
| ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay kit | Promega | E7110 |
| Deposited data | ||
| Single-cell RNA sequencing data | This manuscript | GEO: GSE156524 |
| p63 ChIP-seq data | Kouwenhoven et al. (2010) | GEO: GSE17611 |
| Experimental models: Cell lines | ||
| 3T3-J2 Cell Line | Kerafast | EF3003 |
| 293T cell line | Clontech | 632180 |
| Oligonucleotides | ||
| TaqMan probes | Thermo Fisher Scientific | 4331182 |
| Silencer™ Select siRNAs | Thermo Fisher Scientific | 4427037 |
| Silencer™ Select Negative Control No. 1 siRNA | Thermo Fisher Scientific | 4390843 |
| Software and algorithms | ||
| GraphPad Prism | GraphPad Software | 8 |
| BD FACSDiva | BD Biosciences | 8.0.1 |
| FlowJo | BD Biosciences | 10.6.1 |
| Cell Ranger | 10X Genomics | 2.1 |
| STAR aligner 2.5.1b | Dobin et al., (2013) | https://github.com/alexdobin/STAR |
| R version 3.6.3 | R Foundation | https://www.r-project.org/foundation/ |
| RStudio Desktop 1.1.463 | RStudio | https://www.rstudio.com/products/rstudio-desktop/ |
| Seurat 3.2.3 | Satija et al., (2015) | https://satijalab.org/seurat/ |
| Harmony | 1.0 | |
| R code for single-cell RNA sequencing data | Github | https://github.com/cataalee/ABCB5-limbus/blob/main/ABCB5_limbus.R |
| NIS-elements AR | Nikon | 4.30.01 |
| Image Lab | Bio-Rad | 5.2.1 |
| Adobe Illustrator | Adobe | 24.2.1 |