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. 2022 Jul 1;36(13-14):843–856. doi: 10.1101/gad.349727.122

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© 2022 Sladky et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — Hepatocytes accumulate centrioles during polyploidization. (A) Flow cytometry analysis of hepatocyte ploidy distribution in Centrin-GFP^tg/tg mice. (B) Centriole number in primary hepatocytes from A. N = 5 mice, n = 40–130 cells per mouse, age 10 wk. (C) Representative liver section from Plk4^f/f; Centrin-GFP^tg/tg mice. Scale bar, 10 µm. (D) Number of centriole clusters per cell quantified in liver sections. N = 6 mice, n = 40–65 cells per mouse. (E) The ratio of CEP164 foci over Centrin-GFP foci analyzed in the same liver sections as in D. (F) Representative image of centriole clusters showing examples of the orientation of distal appendages. Scale bar, 10 µm. (G) Quantification of the distance between the two closest positioned CEP164-stained appendages in a centriole cluster. (H) Representative liver section from a Plk4^LKO; Centrin-GFP^tg/tg mouse. Asterisk marks a nonhepatocyte cell containing centrioles. Scale bar, 10 µm. (I) Centriole number quantified by counting Centrin-GFP foci in sections from Plk4^f/f; Centrin-GFP^tg/tg (f/f) and Plk4^LKO; Centrin-GFP^tg/tg (LKO) livers. N = 6 mice, n = 31–65 cells per mouse. (J) Graph showing the correlation between the number of Centrin-GFP foci and nuclear area in Plk4^f/f; Centrin-GFP^tg/tg mice (Pearson R = 0.45–0.84, P < 0.0006) and Plk4^LKO; Centrin-GFP^tg/tg mice (Pearson R = −0.23–0.29, P = 0.18–0.86). Lines represent a linear regression calculated for each mouse. (K,L) Plk4^f/f; Centrin-GFP^tg/tg (f/f, n = 6) and Plk4^LKO; Centrin-GFP^tg/tg (LKO, n = 7) mice were analyzed for the liver to body weight ratio (LW/BW) (K) and serum concentrations of alanine transaminase (ALT), albumin, and total bilirubin (tBili) (L). All data are represented as mean ± SEM. Unpaired two-tailed Student's t-test was performed to determine statistical significance in K and L. Only significant results are indicated.