Figure 3.

ANKRD26 protects against liver damage from chronic CCl₄ treatment. (A) Carbon tetrachloride (CCl₄) treatment scheme: Six-week-old male mice were injected twice per week with 5 mL/kg CCl₄ for 4 wk and analyzed 3 d after the last injection. (B) Liver injury was assessed by measuring the necrotic area in H&E-stained paraffin liver sections from wt (N = 8) and Ankrd26^−/− (A26^−/−, N = 9) mice. (C) Representative H&E-stained liver sections from CCl₄ or corn oil-treated wt and Ankrd26^−/− mice. White dashed lines outline necrotic regions. (D–F) Serum concentrations of alanine transaminase (ALT) (D), aspartate transaminase (AST) (E), and total bilirubin (F) measured in corn oil-treated (Oil) or CCl₄-treated wt and Ankrd26^−/− mice. (G) Liver to body weight ratio (LW/BW) of the same animals. (D–G) For wt control, N = 5. For CCl₄, N = 9. For Ankrd26^−/− control, N = 5. For CCl₄, N = 10. Data are shown as mean ± SEM. Statistical significance was assessed using an unpaired two-tailed Student's t-test (B) or one-way-ANOVA with Sidak's multiple comparisons test (D–G). Only significant results are indicated. (**) P < 0.01, (***) P < 0.001, (****) P < 0.0001.