Figure 1.

Peroxisome biogenesis and structural components are dysregulated at the transcript and protein levels in white matter of patients with MS. A–H, Autopsy normal-appearing subcortical white matter samples from patients with MS and non-MS (control) patients were analyzed by RT-PCR targeting select peroxisome and inflammation-related genes ASPA (A), CD68 (B), PEX3 (C), PEX5L (D), PEX11β (E), ABCD3 (F), HSD17B4 (G), and CAT (H) (n = 7-16). I–L, Immunoblotting was performed on white matter from MS and non-MS controls (n = 4) targeting ASPA (J), PMP70 (K), and catalase (L), with representatives shown (I). β-actin was used as a control for protein loading. Error bars represent standard error of the mean. *p < 0.05; **p < 0.01; unpaired Student's t test. For primary antibodies used for Western blot, immunohistochemistry, and immunofluorescence, see Extended Data Figure 1-1. For PCR primer sequences used in this study, see Extended Data Figure 1-2.