FIG. 6.

Growth curves of E. coli DH5α cells containing P. multocida sialidase genes. Growth in minimal media containing either 0.1% glucose or colominic acid (2-8′ N-acetylneuraminic acid) as the sole carbon source (A) or in 2-3′ sialyl lactose (B), 2-6′ sialyl lactose (C), or fetuin (D). Each substrate provides 0.1% bound sialic acid. ▵, pMMB67HE in DH5α (vector control); ●, pNEU101 (nanH) in DH5α; □, pJRD215 in DH5α (vector control); ⧫, pAH502 (nanB) in DH5α. Each assay was done in triplicate wells; results are plotted as means ± standard deviations.