TABLE 2.

Substrate specificity of purified recombinant NanH for sialyl conjugants

Substrate	Rate of hydrolysisa	Relative rateb
Sialyl α2-3′ lactose	517 ± 29	100
Sialyl α2-6′ lactose	12 ± 1	2
Bovine submaxillary mucin	122 ± 6	23
α-Acid glycoprotein	360 ± 25	70
Apo-transferrin	115 ± 11	22
Fetuin	343 ± 33	66
Colominic acid	5 ± 1.6	1
Ganglioside GM1	1 ± 1	0.2
Ganglioside GD1a	141 ± 1	27
Ganglioside GT1b	70 ± 9	13

^aMicromoles of sialic acid released per milligram of purified sialidase per minute at 37°C. 

^bNormalized with respect to the value for α2-3′ sialyl lactose.