Fig 5. Differences in IAV viral load between B6, ChrX.3^PWD, Chr5^PWD and male and female mice.

Male and female B6 and ChrX.3 mice (A) or B6 and Chr5 mice (B) were infected with PR8 IAV as in Fig 3. On day 6 post-infection, whole lung RNA was extracted as described in the Materials and Methods. Relative expression of IAV NS1 gene RNA was assessed using qRT-PCR and normalized to host housekeeping gene B2m. Data were analyzed by two-way ANOVA with Sidak’s post-hoc comparisons, with P values representing significance of differences as indicated for each comparison.