TABLE 1.
Strains and plasmids
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| C. glutamicum | ||
| ATCC 13032 | Wild type | 1 |
| Δmqo | ATCC 13032 Δmqo | This work |
| Δmdhint | ATCC 13032 mdh::pEMmdhint; Kmr | This work |
| Δmdh | ATCC 13032 Δmdh | This work |
| Δmdhint Δmqo | Δmqo mdh::pEMmdhint; Kmr | This work |
| Δndh | ATCC 13032 ndh::pEMndhint; Kmr | This work |
| Δmdh Δndhint | Δmdh ndh::pEMndhint; Kmr | This work |
| Δmqo Δndhint | Δmqo ndh::pEMndhint; Kmr | This work |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 29 |
| S17-1 | thi-1 F−endAR1 hsdR17 supE44 λ− pro | 34 |
| Plasmids | ||
| pK19MobSacB | Integration vector, Kmr; oriVE. coli oriT sacB | 31 |
| pBluescript II SK(+) | Ampr; lacZ | 33 |
| pEM1 | Integration vector; Kmr; oriVE. coli oriT | 32 |
| pEMmdhint | Internal fragment of mdh in pEM1 | This work |
| pEMndhint | Internal fragment of ndh in pEM1 | This work |