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. 2022 Sep 12;11(1):572–587. doi: 10.1080/21623945.2022.2098583

Figure 5.

Figure 5.

E2F1 overexpression reversed the inhibiting effects of miR-223-3p on lipid accumulating and fibrosis in hepatocyte. a, qRT-PCR measurement of the expression of miR-223-3p in cells treated with miR-223-3p mimic alone or in combination with oe-E2F1. b, Western blot measurement of the expression of E2F1 in cells treated with miR-223-3p mimic alone or in combination with oe-E2F1. c, Lipid deposition determined by Nile red staining in cells treated with miR-223-3p mimic alone or in combination with oe-E2F1. d, TC content in cells treated with miR-223-3p mimic alone or in combination with oe-E2F1. e, TG content in cells treated with miR-223-3p mimic alone or in combination with oe-E2F1. f, Western blot measurement of the protein expression of α-SMA, Coliα1 and TGF-β1 in cells treated with miR-223-3p mimic alone or in combination with oe-E2F1. Measurement data are expressed as mean ± standard deviation. * p < 0.05. Comparison among multiple groups was analysed by one-way ANOVA followed by Tukey’s post hoc test. The cell experiment was repeated three times.