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. 2022 Sep 6;18(9):e1010766. doi: 10.1371/journal.ppat.1010766

Fig 5. The BarA-UvrY TCS regulates the pks island via the Csr system.

Fig 5

(A) RT-qPCR of 16 h macrocolonies of E. coli pTrc99a (pEmpty) and E. coli pTrc99a-CsrA (pCsrA). (B) RT-qPCR of 16 h macrocolonies of E. coli pEmpty and E. coli pCsrB. N = 5–6 independent experiments, each the average of 2 technical replicates. Gene expression was normalized to the gyrA housekeeping gene. Statistical significance was determined by two-way ANOVA with Bonferroni’s test for multiple comparison, **p< 0.01, ***<p< 0.001, ****p< 0.0001, error bars represent SD from the mean. (C) Enumeration of S. aureus from 16 h mixed macrocolonies (1EC:1SA) with either E. coli pEmpty, E. coli pCsrA or E. coli pCsrB. N = 6 independent experiments. (D) Enumeration of S. aureus mixed macrocolonies (1EC:1SA) from 16 h with either E. coli WT, E. coli ClbRmut or E. coli pks deletion mutant. N = 6 independent experiments. Individual data points are indicated with closed circles. (C and D) Statistical significance was determined by Ordinary One-way ANOVA with Dunnett’s test for multiple comparison. ****p< 0.0001, error bars represent SD from the mean. Statistical tests were performed on log-transformed data.