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. 2022 Sep 6;20(9):e3001563. doi: 10.1371/journal.pbio.3001563

Fig 5. The production of eGCs did not require Gsx2 activation.

Fig 5

(A) The experimental procedure for (B-E): TAM was administered at E9.5 to generate control embryos with functional alleles of both Pax6 and Gsx2, a single cKO of Pax6 or a dcKO of Pax6 and Gsx2; brains were analysed at E13.5, E14.5, or E16.5. (B) Colorimetric immunoreactivity for Gsx2 and in situ hybridization for Dlx1 in Pax6 cKO and Pax6 Gsx2 dcKO at E14.5. Scale bar: 0.1 mm. (C) Colorimetric in situ hybridization for Gad1 in Pax6 cKO and Pax6 Gsx2 dcKO at E16.5. Scale bar: 0.1 mm. (D) Fluorescence in situ hybridization for Gsx2 and immunoreactivity for Eomes in Pax6 cKO and Pax6 Gsx2 dcKO at E14.5. Scale bar: 0.01 mm. (E) Colorimetric and fluorescence in situ hybridizations for Robo3, colorimetric immunoreactivity for Gsx2, and fluorescence immunoreactivity for GFP in control, Pax6 cKO, and Pax6 Gsx2 dcKO at E13.5. Scale bars: 0.1 mm and 0.01 mm. (F) The experimental procedure for (G): TAM was administered at E9.5 to generate control embryos with a functional allele of Gsx2 or a cKO of Gsx2 throughout the embryo; brains were analysed at E13.5. (G) Colorimetric in situ hybridizations for Robo3 in control and Gsx2 cKO at E13.5. Scale bar: 0.1 mm. dcKO, double conditional KO; eGC, ectopic GABAergic cell; GFP, green fluorescent protein; Pax6 cKO, Pax6 conditional knockout; TAM, tamoxifen.