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. 2022 Sep 6;18(9):e1010743. doi: 10.1371/journal.ppat.1010743

Fig 1. Individual TRIM25 RING residues required for TRIM25 autoubiquitination.

Fig 1

(A) Alignment of the RING E3 ligases MDM2 (dark blue) and TRIM25 (light blue) in complex with ubiquitin (red) and the E2 UbcH5 (gray), performed using UCSF Chimera [30]. Highlighted in gold (TRIM25) and yellow (MDM2) are homologous residues. PDB: 5MNJ (MDM2), 5EYA (TRIM25). (B) Western blot of 293T cells transfected with FLAG-TRIM25 mutants and HA-ubiquitin (Ub). Lysates were subjected to FLAG IP. Data representative of three independent experiments.