Skip to main content
. 2022 Sep 6;18(9):e1010743. doi: 10.1371/journal.ppat.1010743

Fig 2. TRIM25 co-IP/MS identifies TRIM25 interactors.

Fig 2

(A) Western blot of TRIM25-WT and -R54P doxycycline (dox)-inducible 293T cell lines in the presence of increasing amount of dox (0, 0.001, 0.01, 0.1, 1, and 10 μg/mL). Data are representative of two independent experiments. (B) Schematic of co-IP/MS experiment to identify TRIM25 interactors. (C-D) Volcano plots of proteins significantly enriched over TRIM25 KO background in TRIM25-R54P co-IP/MS in the (C) absence or (D) presence of viral infection. Data representative of two independent experiments. Blue dots represent proteins that were also significantly enriched in TRIM25-WT co-IP and red dots represent proteins that were only enriched in TRIM25-R54P co-IP. Proteins were counted as enriched when log2FC>1.5 and -log10Pvalue>1.3 (Pvalue<0.05). The R package EnhancedVolcano [32] was used to generate volcano plots. (E) Gene ontology terms significantly enriched in all unique TRIM25-WT and TRIM25-R54P interactors. Analysis performed for GO terms in biological processes using DAVID [33,34].