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. 2022 Sep 6;18(9):e1010743. doi: 10.1371/journal.ppat.1010743

Fig 4. TRIM25 interacts with and mono-ubiquitinates UPF1.

Fig 4

(A) Western blot of TRIM25 inducible cells transfected with V5-tagged UPF1 in the presence or absence of 1 μg/mL dox. Lysates were subjected to FLAG IP. Data are representative of three independent experiments. (B-C) Western blot of TRIM25 inducible cells transfected with (B) V5-UPF1 or (C) V5-UPF1 WT and mutants (K281R, K592R) and HA-Ub in the presence of 1 μg/mL dox. Lysates were subjected to V5 IP. Data are representative of two independent experiments for (B) and of three independent experiments for (C).