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. 2022 Sep 6;18(9):e1010743. doi: 10.1371/journal.ppat.1010743

Fig 6. TRIM25 interacts with and polyubiquitinates PABPC4.

Fig 6

(A) Western blot of TRIM25 KO and TRIM25 inducible cells transfected with myc-tagged PABPC4 in the presence of 1 μg/mL dox. Lysates were subjected to a FLAG IP. Data are representative of two independent experiments. (B) Western blot of TRIM25 KO and TRIM25 inducible cells in the presence of 1 μg/mL dox. Lysates were subjected to a FLAG IP. Data are representative of two independent experiments. (C) Western blot of TRIM25 KO and TRIM25 inducible cells treated with 1 μg/mL dox and transfected with myc-PABPC4 and HA-Ub-WT. Lysates were subjected to myc IP. Data are representative of two independent experiments. (D) Western blot of TRIM25-WT inducible cells treated with 1 μg/mL dox and transfected with myc-PABPC4 and HA-Ub-WT, -K48, or -K63. Lysates were subjected to myc IP. Data are representative of two independent experiments.