| strain (Chikungunya virus) |
CHIKV strain 7142.181/25 |
[75] |
|
|
| strain (O’nyong-nyong virus) |
ONNV-GFP |
[76] |
GenBank AF079456
|
SG650 genome |
| strain (Ross River virus) |
RR64-GFP |
[77] |
|
|
| strain (Sindbis virus)
|
Toto1101 |
[78] |
|
|
| strain (Sindbis virus) |
Toto1101/Luc |
[79] |
|
|
| strain (Sindbis virus) |
Toto1101/Luc:ts6 |
[79] |
|
|
| strain (Sindbis virus) |
TE-5’2J/GFP |
[80] |
|
|
| strain (Venezuelan equine encephalitis virus) |
VEEV-GFP |
[81] |
vaccine strain TC-83 |
|
| Antibody |
anti-actin-HRP, mouse monoclonal |
Sigma-Aldrich |
A3854 |
WB (1:20,000) |
| Antibody |
anti-EFP/TRIM25, mouse monoclonal |
BD Biosciences |
610570 |
WB (1:5,000) |
| Antibody |
anti-FLAG, mouse monoclonal |
Sigma-Aldrich |
F1804 |
WB (1:20,000) |
| Antibody |
anti-G3BP1, mouse monoclonal |
Santa Cruz |
sc-365338 |
WB (1:500) |
| Antibody |
anti-G3BP2, rabbit polyclonal |
Assay Biotech |
C18193 |
WB (1:1,000) |
| Antibody |
anti-HA, rat monoclonal |
Roche Life Science |
3F10 |
WB (1:1,000) |
| Antibody |
anti-myc, rabbit polyclonal |
Cell Signaling Technology |
2272S |
WB (1:2,500) |
| Antibody |
anti-NM23A (NME1), rabbit monoclonal |
Abcam |
ab171935 |
WB (1:10,000) |
| Antibody |
anti-PABPC4, rabbit polyclonal |
Proteintech |
14960-1-AP |
WB (1:2,000) |
| Antibody |
anti-UPF1, rabbit monoclonal |
Cell Signaling Technology |
12040 |
WB (1:1,000) |
| Antibody |
anti-V5, mouse monoclonal |
Invitrogen |
MA5-1523 |
WB (1:5,000) |
| Antibody |
donkey anti-rat HRP |
Jackson ImmunoResearch |
712-035-153 |
WB (1:20,000) |
| Antibody |
goat anti-mouse HRP |
Jackson ImmunoResearch |
115-035-146 |
WB (1:20,000) |
| Antibody |
goat anti-rabbit HRP |
Thermo Fisher Scientific |
31462 |
WB (1:20,000) |
| Chemical compound, drug |
poly(I:C) HMW |
InvivoGen |
tlrl-pic |
|
| Chemical compound, drug |
Poly-L-lysine hydrobromide |
Sigma-Aldrich |
P2636 |
|
| Chemical compound, drug |
Roche cOmplete Mini, EDTA-free Protease Inhibitor Cocktail |
Sigma-Aldrich |
11836170001 |
|
| Commercial assay or kit |
DharmaFECT 1 Transfection Reagent |
Horizon Discovery |
T-2001-01 |
|
| Commercial assay or kit |
Dynabeads Protein A for Immunoprecipitation |
Invitrogen |
10-002-D |
|
| Commercial assay or kit |
EZview Red ANTI-FLAG M2 Affinity Gel |
Sigma-Aldrich |
F2426 |
|
| Commercial assay or kit |
EZview Red ANTI-MYC M2 Affinity Gel |
Sigma-Aldrich |
E6654 |
|
| Commercial assay or kit |
KOD Hot Start Master Mix |
Sigma-Aldrich |
71842 |
|
| Commercial assay or kit |
Luna Universal qPCR Master Mix |
New England Biolabs |
M3003X |
|
| Commercial assay or kit |
Mini-PROTEAN TGX Gels, 4–15%, 15 well |
Bio-Rad |
4568086 |
|
| Commercial assay or kit |
NuPAGE MOPS SDS running buffer |
Invitrogen |
NP0001 |
|
| Commercial assay or kit |
ProSignal Full-Range Prestained Protein Ladder |
Genesee Scientific |
83–650 |
|
| Commercial assay or kit |
ProSignal Pico ECL Reagent |
Genesee Scientific |
20-300B |
|
| Commercial assay or kit |
Protoscript II First Strand cDNA Synthesis Kit |
New England Biolabs |
E6560L |
|
| Commercial assay or kit |
Q5 Site-Directed Mutagenesis Kit |
New England Biolabs |
E0552S |
|
| Commercial assay or kit |
Quick-DNA Miniprep-Plus kit |
Zymo Research |
D4068 |
|
| Commercial assay or kit |
Quick-RNA kit |
Zymo Research |
R1055 |
|
| Commercial assay or kit |
QuikChange II XL Site-Directed Mutagenesis Kit |
Agilent |
210518 |
|
| Commercial assay or kit |
RNeasy mini kit |
Qiagen |
74104 |
|
| Commercial assay or kit |
X-tremeGENE9 Transfection Reagent |
Sigma-Aldrich |
6365787001 |
|
| Sequence-based reagent |
DNA primers for molecular cloning |
This work |
S1 Table
|
|
| Sequence-based reagent |
RT-qPCR oligonucleotides |
PrimerBank |
S3 Table
|
https://pga.mgh.harvard.edu/primerbank/
|
| Sequence-based reagent |
siRNA |
Ambion |
S2 Table
|
|
| Software, algorithm |
Database for Annotation, Visualization and Integrated Discovery v6.8 |
Frederick National Laboratory for Cancer Research, Frederick, MD |
|
https://david.ncifcrf.gov/home.jsp
|
| Software, algorithm |
EnhancedVolcano |
Clinical Bioinformatics Research LTD, United Kingdom |
|
https://github.com/kevinblighe/EnhancedVolcano
|
| Software, algorithm |
FlowJo |
BD Biosciences, Franklin, NJ |
|
https://flowjo.com
|
| Software, algorithm |
Geneious Prime (2021.2) |
Biomatters, San Diego, CA |
|
https://geneious.com
|
| Software, algorithm |
GraphPad Prism 9 (v.9.2.0) |
GraphPad Software, San Diego, CA |
|
https://graphpad.com
|
| Software, algorithm |
ImageJ |
National Institutes of Health, Bethesda, MD |
|
https://imagej.net/
|
| Software, algorithm |
RStudio software (v.1.4.1106) |
RStudio, Boston, MA |
|
https://rstudio.com
|
| Software, algorithm |
UCSF Chimera |
University of California, San Francisco, San Francisco, CA |
|
https://www.rbvi.ucsf.edu/chimera/
|
