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. 2022 Sep 9;2022:6450721. doi: 10.1155/2022/6450721

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Copyright © 2022 Zhuo Wu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpression of SPI1 in CCL7-low cells promotes macrophage migration and M2 polarization. mRNA (a) and protein (b) levels of SPI1 and CCL7 in H125^CCL7Low and A549^CCL7High cells after SPI1 overexpression plasmid or shRNA transfection detected by qPCR and WB analyses; (c) number of migrated THP-1 cells examined by crystal violet staining; mRNA (d) and protein (e) levels of CD86 and CD206 in cocultured THP-1 cells examined by qPCR and WB analyses; (f) expression of M1 cytokines (IL-6, IL-1β, and TNF-α) and M2 cytokines (IL-10 and TGF-β) in the culture medium determined by ELISA kits. Repetition = 3. Data are presented as the mean ± SD. ^∗∗p < 0.01.